Aina 4 za Virusi vya Kupumua
Jina la bidhaa
HWTS-RT099- Aina 4 za Virusi vya Kupumua Kiti ya Kugundua Asidi ya Nyuklia (Fluorescence PCR)
Epidemiolojia
Ugonjwa wa Virusi vya Corona 2019, unaojulikana kama "COVID-19", unarejelea nimonia inayosababishwa na2019-nCoVmaambukizi.2019-nCoVni coronavirus ya jenasi β.COVID-19 ni ugonjwa wa kuambukiza kwa papo hapo, na idadi ya watu kwa ujumla huathirika.Kwa sasa, chanzo cha maambukizi ni hasa wagonjwa walioambukizwa na2019-nCoV, na watu walioambukizwa bila dalili wanaweza pia kuwa chanzo cha maambukizi.Kulingana na uchunguzi wa sasa wa epidemiological, kipindi cha incubation ni siku 1-14, zaidi ya siku 3-7.Homa, kikohozi kavu na uchovu ni maonyesho kuu.Wagonjwa wachache walikuwa na dalilis kama vilemsongamano wa pua, pua ya kukimbia, koo, myalgia na kuhara, na kadhalika.
Kituo
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Udhibiti wa Ndani |
Vigezo vya Kiufundi
| Hifadhi | -18 ℃ |
| Maisha ya rafu | miezi 9 |
| Aina ya Kielelezo | Kitambaa cha oropharyngeal |
| Ct | ≤38 |
| LoD | 2019-nCoV: Nakala 300/mLVirusi vya mafua A/Virusi vya mafua B/Virusi vya kupumua vya sinsiti: 500Copies/mL |
| Umaalumu | a) Matokeo ya utendakazi mtambuka yanaonyesha kuwa hakuna athari tofauti kati ya kifaa na virusi vya corona vya binadamu SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, virusi vya parainfluenza aina 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus ya binadamu, enterovirus A, B, C, D, virusi vya mapafu ya binadamu, virusi vya epstein-barr, virusi vya surua, virusi vya human cytomegalo, rotavirus, norovirus, virusi vya parotitis, varisela-zoster virusi, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, moshi aspergillus, candida albicans, candida glabrata glabrata pneumoniae cryptomic genomic acid na pnecoccus binadamu pnecoccus asidi mpya ya binadamu b) Uwezo wa kuzuia mwingiliano: chagua mucin (60mg/mL), 10% (v/v) ya damu na phenylephrine (2mg/mL), oxymetazolini (2mg/mL), kloridi ya sodiamu (pamoja na vihifadhi) (20 mg/mL). ), beclomethasone (20mg/mL), deksamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone asetonidi (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL ), histamini hidrokloridi (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir(500mg/ mL), ritonavir(60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) na tobramycin (0.6mg/mL) mL) kwa ajili ya mtihani wa kuingiliwa, na matokeo yanaonyesha kuwa vitu vinavyoingilia kati na viwango vilivyotajwa hapo juu havina athari ya kuingiliwa kwa matokeo ya mtihani wa pathogens. |
| Vyombo Vinavyotumika | Mfumo wa PCR Uliotumika 7500 wa Wakati Halisi Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Mifumo ya PCR ya Wakati Halisi |
Mtiririko wa Kazi
Chaguo 1.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) na Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006) zinazozalishwa na Jiangsu Macro & Micro -Test Med-Tech Co., Ltd. Kiasi cha sampuli kilichotolewa ni 200μL, na kiwango cha elution kilichopendekezwa ni 80μL.
Chaguo la 2.
QIAamp Viral RNA Mini Kit (52904) inayozalishwa na QIAGEN au Nucleic Acid Extraction or Purification Kit (YDP315-R) inayozalishwa na Tiangen Biotech (Beijing) Co., Ltd. Sampuli ya ujazo iliyotolewa ni 140μL, na ujazo wa elution uliopendekezwa ni 60μL.
